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KMID : 1156220180440050491
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Journal of Environmental Health Sciences
2018 Volume.44 No. 5 p.491 ~ p.501
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Comparison of Human Blood Cadmium Concentrations using Graphite Furnace Atomic Absorption Spectrometry (GF-AAS) and Inductively Coupled Plasma-mass Spectrometry (ICP-MS)
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Kwon Jung-Yeon
Kim Byoung-Gwon
Lim Hyoun-Ju
Seo Jeong-Wook
Kang Min-Kyung
Kim Yu-Mi
Hong Young-Seoub
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Abstract
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Objectives: The aims of this study were to compare concentrations and the correspondence of human blood cadmium by using graphite furnace atomic absorption spectrometry (GF-AAS) and inductively coupled plasmamass spectrometry (ICP-MS), which are representative methods of heavy metal analysis.
Methods: We randomly selected 79 people who agreed to participate in the research project. After confirming the linearity of the calibration curves for GF-AAS and ICP-MS, the concentrations of cadmium in a quality control standard material and blood samples were measured, and the correlation and the degree of agreement were compared.
Results: The detection limit of ICP-MS (IDL: 0.000 μg/L, MDL: 0.06 μg/L) was lower than that of GF-AAS (IDL: 0.085 μg/L, MDL: 0.327 μg/L). The coefficient of variation of the quality control standard material showed stable values for both ICP-MS (clinchek-1: 5.35%, clinchek-2: 6.22%) and GF-AAS (clinchek-1: 7.92%, clinchek-2: 5.22%). Recovery was relatively high for both ICP-MS (clinchek-1: 95.1%, clinchek-2: 92.8%) and GF-AAS (clinchek-1: 91.4%, clinchek-2: 98.8%), with more than 90%. The geometric mean, median, and percentile of blood samples were all similar. The agreement of the two instruments compared with the bias of the analytical values found that about 81% of the analytical values were within ± 30% of the deviation from the ideal reference line (y=0). As a result of the agreement limit, the value included in the confidence interval was about 94%, which shows high agreement.
Conclusion: In this study, we confirmed there was no significant difference in concentrations of a quality control standard material and blood samples. Since ICP-MS showed lower concentrations than GF-AAS at concentrations below the method detection limit of GF-AAS, it is expected that more precise results will be obtained by analyzing blood cadmium with ICP-MS.
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KEYWORD
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Cadmium , ICP-MS , GF-AAS , degree of agreement
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